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diffraction limited wide field epifluorescence microscopy  (Nikon)


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    Nikon diffraction limited wide field epifluorescence microscopy
    Applications of PEPCy tags for imaging cell surface receptors. A . Schematic of membrane protein tracking experiment using TIRF <t>microscopy.</t> AlphaFold predicted structures of PEPCy tags. B . Normalized probability distributions of photons detected per trajectory from single particle analyses between PEPCy and HT targeted to their cognate dyes. (N = 1000-1300 single tracks per sample). C. Super-resolution microscopy of Intimin fused to PEPCy3 expressed on E.coli outer membrane. Schematic of the fusion is shown followed by <t>diffraction-limited</t> DIC and fluorscence images of the cells expressing the same. Maximum intensity projections (MIP) compared with a 2D reconstruction of single molecule localization data. Inset compares a cluster observable in the MIP that is resolved into two clusters in the super-resolved image. D . Time-lapse confocal microscopy of HeLa cells expressing PEPCy3-B2AR labeled using Cy3. Numbers on the left of each image denote the time in minutes. Isoproterenol was added at t = 0 and imaged were acquired every 30s for 1 hour. E . Schematic of simultaneous two color tracking of PEPCy3-B2AR and PEPCy5-B2AR-Ala on the same cell. F . Single molecule trajectories of PEPCy3 (left) and PEPCy5 (right) on a HEK cell, color coded by median trajectory speed. All scale bars in the figure unless mentioned otherwise are 5 µ m. A and E were created with BioRender.com .
    Diffraction Limited Wide Field Epifluorescence Microscopy, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 10098 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/diffraction limited wide field epifluorescence microscopy/product/Nikon
    Average 99 stars, based on 10098 article reviews
    diffraction limited wide field epifluorescence microscopy - by Bioz Stars, 2026-04
    99/100 stars

    Images

    1) Product Images from "PEPCy: Photostable fluoromodules for live cell, super-resolution microscopy of surface proteins"

    Article Title: PEPCy: Photostable fluoromodules for live cell, super-resolution microscopy of surface proteins

    Journal: bioRxiv

    doi: 10.1101/2024.07.03.601615

    Applications of PEPCy tags for imaging cell surface receptors. A . Schematic of membrane protein tracking experiment using TIRF microscopy. AlphaFold predicted structures of PEPCy tags. B . Normalized probability distributions of photons detected per trajectory from single particle analyses between PEPCy and HT targeted to their cognate dyes. (N = 1000-1300 single tracks per sample). C. Super-resolution microscopy of Intimin fused to PEPCy3 expressed on E.coli outer membrane. Schematic of the fusion is shown followed by diffraction-limited DIC and fluorscence images of the cells expressing the same. Maximum intensity projections (MIP) compared with a 2D reconstruction of single molecule localization data. Inset compares a cluster observable in the MIP that is resolved into two clusters in the super-resolved image. D . Time-lapse confocal microscopy of HeLa cells expressing PEPCy3-B2AR labeled using Cy3. Numbers on the left of each image denote the time in minutes. Isoproterenol was added at t = 0 and imaged were acquired every 30s for 1 hour. E . Schematic of simultaneous two color tracking of PEPCy3-B2AR and PEPCy5-B2AR-Ala on the same cell. F . Single molecule trajectories of PEPCy3 (left) and PEPCy5 (right) on a HEK cell, color coded by median trajectory speed. All scale bars in the figure unless mentioned otherwise are 5 µ m. A and E were created with BioRender.com .
    Figure Legend Snippet: Applications of PEPCy tags for imaging cell surface receptors. A . Schematic of membrane protein tracking experiment using TIRF microscopy. AlphaFold predicted structures of PEPCy tags. B . Normalized probability distributions of photons detected per trajectory from single particle analyses between PEPCy and HT targeted to their cognate dyes. (N = 1000-1300 single tracks per sample). C. Super-resolution microscopy of Intimin fused to PEPCy3 expressed on E.coli outer membrane. Schematic of the fusion is shown followed by diffraction-limited DIC and fluorscence images of the cells expressing the same. Maximum intensity projections (MIP) compared with a 2D reconstruction of single molecule localization data. Inset compares a cluster observable in the MIP that is resolved into two clusters in the super-resolved image. D . Time-lapse confocal microscopy of HeLa cells expressing PEPCy3-B2AR labeled using Cy3. Numbers on the left of each image denote the time in minutes. Isoproterenol was added at t = 0 and imaged were acquired every 30s for 1 hour. E . Schematic of simultaneous two color tracking of PEPCy3-B2AR and PEPCy5-B2AR-Ala on the same cell. F . Single molecule trajectories of PEPCy3 (left) and PEPCy5 (right) on a HEK cell, color coded by median trajectory speed. All scale bars in the figure unless mentioned otherwise are 5 µ m. A and E were created with BioRender.com .

    Techniques Used: Imaging, Membrane, Microscopy, Single Particle, Super-Resolution Microscopy, Expressing, Confocal Microscopy, Labeling



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    diffraction limited wide field epifluorescence microscopy  (Nikon)
    99
    Nikon diffraction limited wide field epifluorescence microscopy
    Applications of PEPCy tags for imaging cell surface receptors. A . Schematic of membrane protein tracking experiment using TIRF <t>microscopy.</t> AlphaFold predicted structures of PEPCy tags. B . Normalized probability distributions of photons detected per trajectory from single particle analyses between PEPCy and HT targeted to their cognate dyes. (N = 1000-1300 single tracks per sample). C. Super-resolution microscopy of Intimin fused to PEPCy3 expressed on E.coli outer membrane. Schematic of the fusion is shown followed by <t>diffraction-limited</t> DIC and fluorscence images of the cells expressing the same. Maximum intensity projections (MIP) compared with a 2D reconstruction of single molecule localization data. Inset compares a cluster observable in the MIP that is resolved into two clusters in the super-resolved image. D . Time-lapse confocal microscopy of HeLa cells expressing PEPCy3-B2AR labeled using Cy3. Numbers on the left of each image denote the time in minutes. Isoproterenol was added at t = 0 and imaged were acquired every 30s for 1 hour. E . Schematic of simultaneous two color tracking of PEPCy3-B2AR and PEPCy5-B2AR-Ala on the same cell. F . Single molecule trajectories of PEPCy3 (left) and PEPCy5 (right) on a HEK cell, color coded by median trajectory speed. All scale bars in the figure unless mentioned otherwise are 5 µ m. A and E were created with BioRender.com .
    Diffraction Limited Wide Field Epifluorescence Microscopy, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/diffraction limited wide field epifluorescence microscopy/product/Nikon
    Average 99 stars, based on 1 article reviews
    diffraction limited wide field epifluorescence microscopy - by Bioz Stars, 2026-04
    99/100 stars
    		  Buy from Supplier

    Image Search Results


    Applications of PEPCy tags for imaging cell surface receptors. A . Schematic of membrane protein tracking experiment using TIRF microscopy. AlphaFold predicted structures of PEPCy tags. B . Normalized probability distributions of photons detected per trajectory from single particle analyses between PEPCy and HT targeted to their cognate dyes. (N = 1000-1300 single tracks per sample). C. Super-resolution microscopy of Intimin fused to PEPCy3 expressed on E.coli outer membrane. Schematic of the fusion is shown followed by diffraction-limited DIC and fluorscence images of the cells expressing the same. Maximum intensity projections (MIP) compared with a 2D reconstruction of single molecule localization data. Inset compares a cluster observable in the MIP that is resolved into two clusters in the super-resolved image. D . Time-lapse confocal microscopy of HeLa cells expressing PEPCy3-B2AR labeled using Cy3. Numbers on the left of each image denote the time in minutes. Isoproterenol was added at t = 0 and imaged were acquired every 30s for 1 hour. E . Schematic of simultaneous two color tracking of PEPCy3-B2AR and PEPCy5-B2AR-Ala on the same cell. F . Single molecule trajectories of PEPCy3 (left) and PEPCy5 (right) on a HEK cell, color coded by median trajectory speed. All scale bars in the figure unless mentioned otherwise are 5 µ m. A and E were created with BioRender.com .

    Journal: bioRxiv

    Article Title: PEPCy: Photostable fluoromodules for live cell, super-resolution microscopy of surface proteins

    doi: 10.1101/2024.07.03.601615

    Figure Lengend Snippet: Applications of PEPCy tags for imaging cell surface receptors. A . Schematic of membrane protein tracking experiment using TIRF microscopy. AlphaFold predicted structures of PEPCy tags. B . Normalized probability distributions of photons detected per trajectory from single particle analyses between PEPCy and HT targeted to their cognate dyes. (N = 1000-1300 single tracks per sample). C. Super-resolution microscopy of Intimin fused to PEPCy3 expressed on E.coli outer membrane. Schematic of the fusion is shown followed by diffraction-limited DIC and fluorscence images of the cells expressing the same. Maximum intensity projections (MIP) compared with a 2D reconstruction of single molecule localization data. Inset compares a cluster observable in the MIP that is resolved into two clusters in the super-resolved image. D . Time-lapse confocal microscopy of HeLa cells expressing PEPCy3-B2AR labeled using Cy3. Numbers on the left of each image denote the time in minutes. Isoproterenol was added at t = 0 and imaged were acquired every 30s for 1 hour. E . Schematic of simultaneous two color tracking of PEPCy3-B2AR and PEPCy5-B2AR-Ala on the same cell. F . Single molecule trajectories of PEPCy3 (left) and PEPCy5 (right) on a HEK cell, color coded by median trajectory speed. All scale bars in the figure unless mentioned otherwise are 5 µ m. A and E were created with BioRender.com .

    Article Snippet: Prior to single molecule imaging PEPCy3-Cy3 signal was confirmed by diffraction limited wide-field epifluorescence microscopy, performed on a Nikon Eclipse Ti2 microscope equipped with a super apochromat objective (PlanApo, 100x, 1.45 N.A., oil immersion, Nikon) and scientific cMOS camera (Prime95B, Photometrix).

    Techniques: Imaging, Membrane, Microscopy, Single Particle, Super-Resolution Microscopy, Expressing, Confocal Microscopy, Labeling